Screening and Verifying Mycotoxins in Food with Q-TOF LC/MS and an Accurate Mass Library Authors
نویسندگان
چکیده
This application note describes the creation of an accurate mass library for mycotoxins and related metabolites and its application for the screening of mycotoxins in food. An Agilent 1290 Infinity LC coupled to an Agilent 6550 iFunnel Q-TOF LC/MS was operated in positive and negative electrospray with dual-spray Agilent Jet Stream Technology. Accurate mass spectra were acquired for a large collection of mycotoxins and related metabolites in one or both ionization modes and for all relevant ion species. Three different matrices were extracted and spiked with 44 indicator compounds. Samples were analyzed using target MS/MS and All Ions MS/MS acquisition. This work demonstrates the value of both acquisition modes, combined with an efficient data analysis workflow and the Mycotoxins and Related Metabolites Personal Compound Database and Library (PCDL), for the screening and verification of mycotoxins in complex matrices.
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